Plasmid

Part:BBa_K2141000:Design

Designed by: Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun   Group: iGEM16_Istanbul_Tech   (2016-10-04)


pET28a(+) expression plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 335
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 192
    Illegal NheI site found at 231
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 192
    Illegal BglII site found at 401
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 335
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 335
    Illegal NgoMIV site found at 433
    Illegal NgoMIV site found at 2021
    Illegal NgoMIV site found at 2181
    Illegal NgoMIV site found at 5228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3101


Design Notes

We tried to eliminate unwanted C- and N- terminal tags by choosing proper restriction sites.


Source

It is a commercially available expression plasmid.

References